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gary dunny  (Addgene inc)


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    Addgene inc gary dunny
    Gary Dunny, supplied by Addgene inc, used in various techniques. Bioz Stars score: 88/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pmsp3535/pMSP3535+(Plasmid+%2346886)/pmc11190166-142-17-19
    Average 88 stars, based on 9 article reviews
    gary dunny - by Bioz Stars, 2026-09
    88/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Cross-Genus "Boot-Up" of Synthetic Bacteriophage in Staphylococcus aureus by Using a New and Efficient DNA Transformation Method.
    Article Snippet: .. Plasmid pGF35, pMSP3535 (Addgene plasmid number 46886) (78) with GFP inserted, was provided by Roy Stevens, Kornberg School of Dentistry, Temple University, USA. pCAS9counter was a gift from Steve Salipante (Addgene plasmid number 107192) (79). ..

    Article Title: Extracellular Electron Transfer Powers Enterococcus faecalis Biofilm Metabolism
    Article Snippet: .. E. faecalis strains harboring pMSP3535 (source, Gary M. Dunny; Addgene plasmid 46886) or pMSP3535:: ldh1 (source, Axel Hartke [ ]) were selected for with 300 μg/ml erythromycin and induced with 5-μg/ml nisin-supplemented BHI. .. BHI was supplied by Becton, Dickinson and Company, Franklin Lakes, NJ.

    Article Title: Linoleic Acids Overproducing Lactobacillus casei Limits Growth, Survival, and Virulence of Salmonella Typhimurium and Enterohaemorrhagic Escherichia coli
    Article Snippet: An aliquot of 6 mL cell suspension containing 1 × 10 6 cells were transferred into 25 cm 2 flask (Greiner Bio-One, Monroe, NC, United States) and cultured at standard condition for 24–30 h. After time, the cell monolayer was washed for three times with RPMI for further bacterial infection. .. Plasmid pJET and E . coli DH5α were purchased from Thermo Fisher Scientific (Waltham, MA, United States), pDS132 and E . coli β2155 were donated by Dr. Fidelma Boyd (Delaware University, Newark, DE, United States), and pMSP3535 were purchased from Addgene (Cambridge, MA, United States). .. LC-WT and L . rhamnosus GG (ATCC 53103) were harvested from overnight culture in MRS broth, followed by three times sub-culture on MRS agar plate at 37°C for 24 h in the presence of 5% CO 2 incubator.

    Article Title: Expanding the genetic toolbox for the obligate human pathogen Streptococcus pyogenes
    Article Snippet: .. For some of the PCRs, we used the following plasmids as template: pEU8517 , pMSP3535 donated by Gary Dunny (Addgene plasmid # 46886) , pPEPY-PF6-lacI (Addgene plasmid # 85589) and pJWV102-PL-dCas9 (Addgene plasmid # 85588) donated by Jan-Willem Veening ( ). ..

    Article Title: Linoleic Acids Overproducing Lactobacillus casei Limits Growth, Survival, and Virulence of Salmonella Typhimurium and Enterohaemorrhagic Escherichia coli .
    Article Snippet: .. Plasmid pJET and E. coli DH5α were purchased from Thermo Fisher Scientific (Waltham, MA, United States), pDS132 and E. coli β2155 were donated by Dr. Fidelma Boyd (Delaware University, Newark, DE, United States), and pMSP3535 were purchased from Addgene (Cambridge, MA, United States). .. LCWT and L. rhamnosus GG (ATCC 53103) were harvested from overnight culture in MRS broth, followed by three times subculture on MRS agar plate at 37◦C for 24 h in the presence of 5% CO2 incubator.

    Article Title: Expanding the genetic toolbox for the obligate human pathogen Streptococcus pyogenes
    Article Snippet: .. For some of the PCRs, we used the following plasmids as template: pEU8517 , pMSP3535 donated by Gary Dunny (Addgene plasmid # 46886) , pPEPY-PF6-lacI (Addgene plasmid # 85589) and pJWV102-PL-dCas9 (Addgene plasmid # 85588) donated by Jan-Willem Veening ( ). ..

    Article Title: Extracellular Electron Transfer Powers Enterococcus faecalis Biofilm Metabolism
    Article Snippet: .. E. faecalis strains harboring pMSP3535 (source, Gary M. Dunny; Addgene plasmid 46886) or pMSP3535::ldh1 (source, Axel Hartke [71]) were selected for with 300 g/ml erythromycin and induced with 5- g/ml nisin-supplemented BHI. .. BHI was supplied by Becton, Dickinson and Company, Franklin Lakes, NJ.

    Modification:

    Article Title: The Heterodimeric ABC Transporter EfrCD Mediates Multidrug Efflux in Enterococcus faecalis
    Article Snippet: .. These two complementation vectors were modified from 202 pMSP3535 (Addgene #46886). ..



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    The parental strain E. faecalis JH2-2 ( 1 ), and its derivative mutants Δ sigV ( 2 ), SAS <t>pMSP3535</t> ( 3 ), and SAS pMSP3535- sigV ( 4 ) were grown on LB solid medium without lysozyme ( A ) or supplemented with 20 mg/ml of lysozyme ( B ). Five µl of 10 −2 dilution of overnight cultures adjusted to OD 600 = 1 in physiological water were plated on solid medium, incubated at 37°C for 48 hours and photographed.
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    Image Search Results


    The parental strain E. faecalis JH2-2 ( 1 ), and its derivative mutants Δ sigV ( 2 ), SAS pMSP3535 ( 3 ), and SAS pMSP3535- sigV ( 4 ) were grown on LB solid medium without lysozyme ( A ) or supplemented with 20 mg/ml of lysozyme ( B ). Five µl of 10 −2 dilution of overnight cultures adjusted to OD 600 = 1 in physiological water were plated on solid medium, incubated at 37°C for 48 hours and photographed.

    Journal: PLoS ONE

    Article Title: The Extracytoplasmic Function Sigma Factor SigV Plays a Key Role in the Original Model of Lysozyme Resistance and Virulence of Enterococcus faecalis

    doi: 10.1371/journal.pone.0009658

    Figure Lengend Snippet: The parental strain E. faecalis JH2-2 ( 1 ), and its derivative mutants Δ sigV ( 2 ), SAS pMSP3535 ( 3 ), and SAS pMSP3535- sigV ( 4 ) were grown on LB solid medium without lysozyme ( A ) or supplemented with 20 mg/ml of lysozyme ( B ). Five µl of 10 −2 dilution of overnight cultures adjusted to OD 600 = 1 in physiological water were plated on solid medium, incubated at 37°C for 48 hours and photographed.

    Article Snippet: For this purpose, SigV was overproduced using SAS pMSP3535 -sigV and SAS pMSP3535 (control) strains under appropriate condition of nisin (0.5 µg/ml) induction and we analyzed the transcription of these genes using RT-qPCR.

    Techniques: Incubation

    Bacterial strains and plasmids.

    Journal: PLoS ONE

    Article Title: The Extracytoplasmic Function Sigma Factor SigV Plays a Key Role in the Original Model of Lysozyme Resistance and Virulence of Enterococcus faecalis

    doi: 10.1371/journal.pone.0009658

    Figure Lengend Snippet: Bacterial strains and plasmids.

    Article Snippet: For this purpose, SigV was overproduced using SAS pMSP3535 -sigV and SAS pMSP3535 (control) strains under appropriate condition of nisin (0.5 µg/ml) induction and we analyzed the transcription of these genes using RT-qPCR.

    Techniques: Mutagenesis

    Oligonucleotide primers used in this study.

    Journal: PLoS ONE

    Article Title: The Extracytoplasmic Function Sigma Factor SigV Plays a Key Role in the Original Model of Lysozyme Resistance and Virulence of Enterococcus faecalis

    doi: 10.1371/journal.pone.0009658

    Figure Lengend Snippet: Oligonucleotide primers used in this study.

    Article Snippet: For this purpose, SigV was overproduced using SAS pMSP3535 -sigV and SAS pMSP3535 (control) strains under appropriate condition of nisin (0.5 µg/ml) induction and we analyzed the transcription of these genes using RT-qPCR.

    Techniques: Clone Assay

    The parental strain E. faecalis JH2-2 ( 1 ), and its derivative mutants Δ sigV ( 2 ), SAS pMSP3535 ( 3 ), and SAS pMSP3535- sigV ( 4 ) were grown on LB solid medium without lysozyme ( A ) or supplemented with 20 mg/ml of lysozyme ( B ). Five µl of 10 −2 dilution of overnight cultures adjusted to OD 600 = 1 in physiological water were plated on solid medium, incubated at 37°C for 48 hours and photographed.

    Journal: PLoS ONE

    Article Title: The Extracytoplasmic Function Sigma Factor SigV Plays a Key Role in the Original Model of Lysozyme Resistance and Virulence of Enterococcus faecalis

    doi: 10.1371/journal.pone.0009658

    Figure Lengend Snippet: The parental strain E. faecalis JH2-2 ( 1 ), and its derivative mutants Δ sigV ( 2 ), SAS pMSP3535 ( 3 ), and SAS pMSP3535- sigV ( 4 ) were grown on LB solid medium without lysozyme ( A ) or supplemented with 20 mg/ml of lysozyme ( B ). Five µl of 10 −2 dilution of overnight cultures adjusted to OD 600 = 1 in physiological water were plated on solid medium, incubated at 37°C for 48 hours and photographed.

    Article Snippet: For this purpose, SigV was overproduced using SAS pMSP3535 -sigV and SAS pMSP3535 (control) strains under appropriate condition of nisin (0.5 µg/ml) induction and we analyzed the transcription of these genes using RT-qPCR.

    Techniques: Incubation

    Bacterial strains and plasmids.

    Journal: PLoS ONE

    Article Title: The Extracytoplasmic Function Sigma Factor SigV Plays a Key Role in the Original Model of Lysozyme Resistance and Virulence of Enterococcus faecalis

    doi: 10.1371/journal.pone.0009658

    Figure Lengend Snippet: Bacterial strains and plasmids.

    Article Snippet: For this purpose, SigV was overproduced using SAS pMSP3535 -sigV and SAS pMSP3535 (control) strains under appropriate condition of nisin (0.5 µg/ml) induction and we analyzed the transcription of these genes using RT-qPCR.

    Techniques: Mutagenesis

    Oligonucleotide primers used in this study.

    Journal: PLoS ONE

    Article Title: The Extracytoplasmic Function Sigma Factor SigV Plays a Key Role in the Original Model of Lysozyme Resistance and Virulence of Enterococcus faecalis

    doi: 10.1371/journal.pone.0009658

    Figure Lengend Snippet: Oligonucleotide primers used in this study.

    Article Snippet: For this purpose, SigV was overproduced using SAS pMSP3535 -sigV and SAS pMSP3535 (control) strains under appropriate condition of nisin (0.5 µg/ml) induction and we analyzed the transcription of these genes using RT-qPCR.

    Techniques: Clone Assay