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gary dunny  (Addgene inc)


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    Addgene inc gary dunny
    Gary Dunny, supplied by Addgene inc, used in various techniques. Bioz Stars score: 88/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gary dunny/product/Addgene inc
    Average 88 stars, based on 8 article reviews
    gary dunny - by Bioz Stars, 2026-05
    88/100 stars

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    The parental strain E. faecalis JH2-2 ( 1 ), and its derivative mutants Δ sigV ( 2 ), SAS pMSP3535 ( 3 ), and SAS pMSP3535- sigV ( 4 ) were grown on LB solid medium without lysozyme ( A ) or supplemented with 20 mg/ml of lysozyme ( B ). Five µl of 10 −2 dilution of overnight cultures adjusted to OD 600 = 1 in physiological water were plated on solid medium, incubated at 37°C for 48 hours and photographed.

    Journal: PLoS ONE

    Article Title: The Extracytoplasmic Function Sigma Factor SigV Plays a Key Role in the Original Model of Lysozyme Resistance and Virulence of Enterococcus faecalis

    doi: 10.1371/journal.pone.0009658

    Figure Lengend Snippet: The parental strain E. faecalis JH2-2 ( 1 ), and its derivative mutants Δ sigV ( 2 ), SAS pMSP3535 ( 3 ), and SAS pMSP3535- sigV ( 4 ) were grown on LB solid medium without lysozyme ( A ) or supplemented with 20 mg/ml of lysozyme ( B ). Five µl of 10 −2 dilution of overnight cultures adjusted to OD 600 = 1 in physiological water were plated on solid medium, incubated at 37°C for 48 hours and photographed.

    Article Snippet: For this purpose, SigV was overproduced using SAS pMSP3535 -sigV and SAS pMSP3535 (control) strains under appropriate condition of nisin (0.5 µg/ml) induction and we analyzed the transcription of these genes using RT-qPCR.

    Techniques: Incubation

    Bacterial strains and plasmids.

    Journal: PLoS ONE

    Article Title: The Extracytoplasmic Function Sigma Factor SigV Plays a Key Role in the Original Model of Lysozyme Resistance and Virulence of Enterococcus faecalis

    doi: 10.1371/journal.pone.0009658

    Figure Lengend Snippet: Bacterial strains and plasmids.

    Article Snippet: For this purpose, SigV was overproduced using SAS pMSP3535 -sigV and SAS pMSP3535 (control) strains under appropriate condition of nisin (0.5 µg/ml) induction and we analyzed the transcription of these genes using RT-qPCR.

    Techniques: Mutagenesis

    Oligonucleotide primers used in this study.

    Journal: PLoS ONE

    Article Title: The Extracytoplasmic Function Sigma Factor SigV Plays a Key Role in the Original Model of Lysozyme Resistance and Virulence of Enterococcus faecalis

    doi: 10.1371/journal.pone.0009658

    Figure Lengend Snippet: Oligonucleotide primers used in this study.

    Article Snippet: For this purpose, SigV was overproduced using SAS pMSP3535 -sigV and SAS pMSP3535 (control) strains under appropriate condition of nisin (0.5 µg/ml) induction and we analyzed the transcription of these genes using RT-qPCR.

    Techniques: Clone Assay

    The parental strain E. faecalis JH2-2 ( 1 ), and its derivative mutants Δ sigV ( 2 ), SAS pMSP3535 ( 3 ), and SAS pMSP3535- sigV ( 4 ) were grown on LB solid medium without lysozyme ( A ) or supplemented with 20 mg/ml of lysozyme ( B ). Five µl of 10 −2 dilution of overnight cultures adjusted to OD 600 = 1 in physiological water were plated on solid medium, incubated at 37°C for 48 hours and photographed.

    Journal: PLoS ONE

    Article Title: The Extracytoplasmic Function Sigma Factor SigV Plays a Key Role in the Original Model of Lysozyme Resistance and Virulence of Enterococcus faecalis

    doi: 10.1371/journal.pone.0009658

    Figure Lengend Snippet: The parental strain E. faecalis JH2-2 ( 1 ), and its derivative mutants Δ sigV ( 2 ), SAS pMSP3535 ( 3 ), and SAS pMSP3535- sigV ( 4 ) were grown on LB solid medium without lysozyme ( A ) or supplemented with 20 mg/ml of lysozyme ( B ). Five µl of 10 −2 dilution of overnight cultures adjusted to OD 600 = 1 in physiological water were plated on solid medium, incubated at 37°C for 48 hours and photographed.

    Article Snippet: For this purpose, SigV was overproduced using SAS pMSP3535 -sigV and SAS pMSP3535 (control) strains under appropriate condition of nisin (0.5 µg/ml) induction and we analyzed the transcription of these genes using RT-qPCR.

    Techniques: Incubation

    Bacterial strains and plasmids.

    Journal: PLoS ONE

    Article Title: The Extracytoplasmic Function Sigma Factor SigV Plays a Key Role in the Original Model of Lysozyme Resistance and Virulence of Enterococcus faecalis

    doi: 10.1371/journal.pone.0009658

    Figure Lengend Snippet: Bacterial strains and plasmids.

    Article Snippet: For this purpose, SigV was overproduced using SAS pMSP3535 -sigV and SAS pMSP3535 (control) strains under appropriate condition of nisin (0.5 µg/ml) induction and we analyzed the transcription of these genes using RT-qPCR.

    Techniques: Mutagenesis

    Oligonucleotide primers used in this study.

    Journal: PLoS ONE

    Article Title: The Extracytoplasmic Function Sigma Factor SigV Plays a Key Role in the Original Model of Lysozyme Resistance and Virulence of Enterococcus faecalis

    doi: 10.1371/journal.pone.0009658

    Figure Lengend Snippet: Oligonucleotide primers used in this study.

    Article Snippet: For this purpose, SigV was overproduced using SAS pMSP3535 -sigV and SAS pMSP3535 (control) strains under appropriate condition of nisin (0.5 µg/ml) induction and we analyzed the transcription of these genes using RT-qPCR.

    Techniques: Clone Assay